Fabrication of multi-well platform with electrical stimulation for efficient myogenic commitment of C2C12 cells
Joohyun Song,Eunjee A. Lee,Seungwoo Cha,Insun Kim,Yonghoon Choi,Nathaniel S. Hwang
Abstract
To engineer tissue-like structures, cells are required to organize themselves into threedimensional networks that mimic the native tissue micro-architecture. Here, we present agarose-based multiwell platform incorporated with electrical stimulation to build skeletal muscle-like tissues in a facile and highly reproducible fashion. Electrical stimulation of C2C12 cells encapsulated in collagen/matrigel hydrogels facilitated the formation 3D muscle tissues. Consequently, we confirmed the transcriptional upregulations of myogenic related genes in the electrical stimulation group compared to non-stimulated control group in our multi-well 3D culture platform. Given the robust fabrication, engineered muscle tissues in multi-well platform may find their use in high-throughput biological studies drug screenings.
Joohyun Song, Eunjee A. Lee, Seungwoo Cha, Insun Kim, Yonghoon Choi and Nathaniel S. Hwang: School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742, Republic of Korea
Nathaniel S. Hwang: Institute of Chemical Process, Seoul National University, Seoul 151-742, Republic of Korea
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