One-step phyto-mediated fabrication of silver nanoparticles and its anti-microbial properties

Velmurugan Palanivel , Sung-Chul Hong , Veera Ravi Arumugam , Sivakumar Subpiramaniyam , Pyong-In Yi , Seong-Ho Jang , Jeong-Min Suh , Eun-Sang Jung , Je-Sung Park

Abstract

This manuscript describes the one-step eco-friendly green fabrication of silver nanoparticles (AgNPs) through the in-situ bio-reduction of an aqueous solution of silver nitrate using Syzygium aromaticum leaf extract. UV–vis spectroscopy shows a characteristic SPR peak around 442 nm. FTIR spectroscopy showed that the AgNPs were capped with bioactive phyto-molecules. TEM images revealed oval and spherical particles with a mean diameter of ~12.6 nm. XRD analysis revealed crystalline and face-cantered cubic AgNPs. The phytosynthesized AgNPs showed broad-spectrum anti-microbial activity against two foodborne pathogenic bacteria, Listeria monocytogenes and Staphylococcus aureus. The AgNPs showed a prominent ability to inhibit biofilms formed by L. monocytogenes and S. aureus in laboratory conditions through a crystal violet assay. The results suggest that the AgNPs could be a novel nanotool to develop effective antimicrobial and anti-biofilm agents in food preservation.

Key Words

anti-bacterial; anti-biofilm; characterization; nanoparticles; phytosynthesis

Address

Velmurugan Palanivel — Centre for Research Bharath Institute of Higher Education and Research 173, Agaram Main Road, Selaiyur, Tambaram, Chennai - 600073, Tamil Nadu, India/ Division of Nanomaterials for Biological and Environmental Application, Department of Biotechnology, Science Campus, Alagappa University, Karaikudi 630002, Tamilnadu, India
Sung-Chul Hong, Sivakumar Subpiramaniyam, Pyong-In Yi, Seong-Ho Jang, Jeong-Min Suh, Eun-Sang Jung and Je-Sung Park — Department of Bioenvironmental Energy, College of Natural Resource and Life Science, Pusan National University, Miryang 50463, Republic of Korea
Veera Ravi Arumugam — Centre for Research Bharath Institute of Higher Education and Research 173, Agaram Main Road, Selaiyur, Tambaram, Chennai - 600073, Tamil Nadu, India

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