Fe3O4 magnetic nanoparticles provide a novel alternative strategy for Staphylococcus aureus bone infection

Youliang Ren , Jin Yang , Jinghui Zhang , Xiao Yang , Lei Shi , Dajing Guo , Yuanyi Zheng , Haitao Ran , Zhongliang Deng , Lei Chu

Abstract

Due to its biofilm formation and colonization of the osteocyte-lacuno canalicular network (OLCN), Staphylococcus aureus (S.aureus) implant-associated bone infection (SIABI) is difficult to cure thoroughly, and may occur recurrently subsequently after a long period dormant. It is essential to explore an alternative therapeutic strategy that can eradicate the pathogens in the infected foci. To address this, the polymethylmethacrylate (PMMA) bone cement and Fe3O4 nanoparticles compound cylinder were developed as implants based on their size and mechanical properties for the alternative magnetic field (AMF) induced thermal ablation, The PMMA mixed with optimized 2% Fe3O4 nanoparticles showed an excellent antibacterial efficacy in vitro. It was evaluated by the CFU, CT scan and histopathological staining on a rabbit 1-stage transtibial screw model. The results showed that on week 7, the CFU of infected soft tissue and implants, and the white blood cells (WBCs) of the PMMA+2% Fe3O4+AMF group decreased significantly from their controls (p

Key Words

biofilm; bone infection; Fe3O4 magnetic nanoparticles; OLCN; Staphylococcus aureus

Address

Youliang Ren, Jin Yang, Xiao Yang, Lei Shi, Zhongliang Deng and Lei Chu — Department of Orthopaedics, Second Affiliated Hospital of Chongqing Medical University, 76 Linjiang Road, Yuzhong Distinct, Chongqing, 400010, P. R. China
Jinghui Zhang — Key Laboratory of Diagnostic Medicine designated by the Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University Chongqing, Yuzhong Distinct, 400016, P. R. China
Dajing Guo — Department of Radiology, The Second Affiliated Hospital of Chongqing Medical University, 76 Linjiang Road, Yuzhong Distinct, Chongqing, 400010, P. R. China
Yuanyi Zheng — Shanghai Institute of Ultrasound in Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 Yishan Road, Xuhui Distinct, Shanghai, 200233, P. R. China
Haitao Ran — Chongqing Key Laboratory of Ultrasound Molecular Imaging, Second Affiliated Hospital of Chongqing Medical University, 76 Linjiang Road, Yuzhong Distinct, Chongqing, 400010, P. R. China

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