Many studies have shown that Mg-Nd-Zn-Zr (abbreviated as JDBM) alloy has good biocompatibility and biodegradability as well as promotion of cell adhesion, proliferation and differentiation, and Wnt/β-catenin signaling pathway may play a unique role in joint tissue by controlling the function of chondrocytes, osteoblasts and synoviocytes. However, it is not clear whether the JDBM alloy induces osteochondral repair through Wnt/β-catenin signaling pathway. This study aims to verify that JDBM alloy can repair osteochondral defects in rats, which is realized by Wnt/β-catenin signaling pathway. In this study, the osteochondral defect model of the right femoral condyle non-weight-bearing area in rats was established and randomly divided into three groups: Control group, JDBM alloy implantation group and JDBM alloy implantation combined with signaling pathway inhibitor drug ICRT3 injection. It was found that after JDBM alloy implantation, the bone volume fraction (BVF) became larger, the bone trabeculae were increased, the relative expression of osteogenesis gene Runx2, Bmp2, Opn, Ocn and chondrogenesis gene Collagen II, Aggrecan were increased, and the tissue repair was obvious by HE and Masson staining, which could be inhibited by ICRT3.
Key Words
biodegradable magnesium alloy; western blot semi-quantitative analysis; Wnt/β-catenin signaling pathway
Address
Kexin Zhao — Department of Bone & Joint Surgery, Peking University Shenzhen Hospital, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center, Guangdong province China/ Department of Bone & Joint Surgery, Peking University Shenzhen Hospital, Shenzhen, PR China, 518036/ National & Local Joint Engineering Research Center of Orthopaedic Biomaterials, Peking University Shenzhen Hospital, Shenzhen, PR China, 518036
Yingqi Chen, Fei Yu, Weng Jian and Hui Zeng — Department of Bone & Joint Surgery, Peking University Shenzhen Hospital, Shenzhen, PR China, 518036/ National & Local Joint Engineering Research Center of Orthopaedic Biomaterials, Peking University Shenzhen Hospital, Shenzhen, PR China, 518036
Ming Zheng — Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Health Science Center, Peking University, Beijing, China,100191/ Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Beijing, China
PDF Viewer
Preview is limited to the first 3 pages. Sign in to access the full PDF.
