Wind tunnel test is one of the most important means to study the flutter performance of bridges, but there are blockage effects in flutter test due to the size limitation of the wind tunnel. On the other hand, the size of computational domain can be defined by users in the numerical simulation. This paper presents a study on blockage effects of a simplified box girder by computation fluid dynamics (CFD) simulation, the blockage effects on the aerodynamic characteristics and flutter performance of a long-span suspension bridge are studied. The results show that the aerodynamic coefficients and the absolute value of mean pressure coefficient increase with the increase of the blockage ratio. And the aerodynamic coefficients can be corrected by the mean wind speed in the plane of leading edge of model. At each angle of attack, the critical flutter wind speed decreases as the blockage ratio increases, but the difference is that bending-torsion coupled flutter and torsional flutter occur at lower and larger angles of attack respectively. Finally, the correction formula of critical wind speed at 0o angle of attack is given, which can provide reference for wind resistance design of streamlined box girders in practical engineering.