Evaluation of antibacterial activity and cytotoxic effects of green AgNPs against Breast Cancer Cells (MCF 7)

Dhandapani Kayal Vizhi, Nookala Supraja, Anbumani Devipriya, Naga Venkata Krishna Vara Prasad Tollamadugu and Ranganathan Babujanarthanam

Abstract

The present work reports a facile, rapid and an eco-friendly method for the synthesis of silver nanoparticles using Luffa acutangula (L. acutangula) leaves extract and their antibacterial and cytotoxic effects. The synthesized silver nanoparticles (AgNPs) were characterized by UV-Visible spectroscopy (UVVis), Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction analysis (XRD). Additionally the topography, morphology and the elemental composition of the particles were determined by Scanning Electron Microscopy (SEM) and Energy dispersive spectrophotometric (EDS) technique and the measured particle sizes from SEM micrographs are in the range of 12.5 to 24.5nm. The in-vitro antimicrobial activity of the synthesized nanoparticles was high against gram positive Staphylococcus aureus and moderate against gram negative Escherichia coli and Pseudomonas aeruginosa strains. Further, the cytotoxic effects of synthesized AgNPs were evaluated against Human Breast Cancer (MCF 7) cell line.

Key Words

silver nanoparticles; Luffa acutangula; antibacterial activity; cytotoxic effect; cancer cells

Address

Dhandapani Kayal Vizhi: Department of Biotechnology, Thiruvalluvar University, Serkkadu, Vellore 632 115, TN, India Nookala Supraja: Nanotechnology Laboratory, Institute of Frontier Technology, Regional Agricultural Research Station, Acharya N G Ranga Agricultural University, Tirupathi 517 502, AP, India Anbumani Devipriya: Department of Biotechnology, Thiruvalluvar University, Serkkadu, Vellore 632 115, TN, India Naga Venkata Krishna Vara Prasad Tollamadugu: Nanotechnology Laboratory, Institute of Frontier Technology, Regional Agricultural Research Station, Acharya N G Ranga Agricultural University, Tirupathi 517 502, AP, India Ranganathan Babujanarthanam: Department of Biotechnology, Thiruvalluvar University, Serkkadu, Vellore 632 115, TN, India

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